electroporation instrument  (Bio-Rad)

Journal: STAR Protocols

Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

doi: 10.1016/j.xpro.2026.104353

Figure Lengend Snippet: Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

Techniques: Plasmid Preparation, Transformation Assay, Marker, Amplification, Electroporation, Homologous Recombination, Selection, Mutagenesis

Journal: STAR Protocols

Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

doi: 10.1016/j.xpro.2026.104353

Figure Lengend Snippet: Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

Techniques: Clone Assay, Transformation Assay, Electroporation, Negative Control, Positive Control

Journal: BMC molecular and cell biology

Article Title: Differentiation of Wharton's jelly-derived mesenchymal stromal cells into hepatocyte-like cells using a refined method.

doi: 10.1186/s12860-025-00534-y

Figure Lengend Snippet: Fig. 4 Evaluating of the best conditions of the mimic miRNAs’ complexes (7 or 4 mimic miRs complex) at different concentrations of 100 pM (A) or 200 pM (B) using electroporation. MSCs (mesenchymal stromal cells); NC (non-coding miRNAs); The asymmetry in the error bars is due to the logarithmic scale used for the Y-axis; X-axis represents the different HLCs produced by various methods and The Y-axis is fold change of gene expression

Article Snippet: Additionally, a Gene Pulser Xcell electroporation instrument (BIO-RAD, USA) was employed for this investigation.

Techniques: Electroporation, Produced, Gene Expression